fluorometer
AELAB Fluorometer
Fluorescence spectroscopy is mainly used to determine the content of certain components in biological samples, analysis of biotechnology and immunotechnology, such as the determination of deoxyribose and deoxyribonucleic acid, DNA, antibodies, antigens, and other aspects of research.Fluorometers are used for precise quantitation of biological molecules including nucleic acids and proteins in microliter (μl) samples.
AE-Fluo-100A,AE-Fluo-100B,AE-Fluo-100C
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Quantity:

AELABFluorometer

Our nano series Micro-Spectrophotometer measure the concentration and purity of the sample, because it is able to measure the absorbance of the whole ingredient(such as DNA, RNA, oligos and so on) of the sample at 260nm, that may make the read inaccuracy. While the fluorometer measure the fluorescence intensity of the fluorescent reagent which combine with the target molecule to read the concentration of sample. Generally, the read is lower than Nano series products but much accuracy than it

The sensitivity of Nano series products are above 5ng/ul, and while the fluorometer is 0.5pg/ul in measuring DNA concentration. It is important and necessary for the most applications to read the lower and accuracy concentration of the sample. Using the Fluo-100 fluorometer with the quantitation kit can measure the sample concentration rapidly, sensitively and accurately

AE-Fluo-100 Simple, easy to use and applications

TheAE-Fluo-100 is a dual-channel fluorometer, it provides high-sensitive fluorescent detection when quantifying nucleic acids and proteins, simple and cost to operate Applications:

  1. Thesampleisscarceanddifficult inprocessing
  2. The low quantityofDNA, RNA, orprotein after extraction
  3. Thesamplewillbeusedintheexpensive experiments:qPCR,PCRcloning,transfection,the next generation sequencing and etc.

AE-Fluo-100 Highlights

+Simple – 4.3 inch touch screen, small and easy to use
+Easy measurement within 3 sec for DNA, RNA, and protein
+High sensitivity – Lowest DNA detection limit is 0.5pg/ul
+Twooptical channels – Equipped with two fluorescence channels for nucleic acid, protein quantitation in one detection
+Linear Dynamic Range--Five orders of magnitude.
+Opening system--You can match the instrument with the reagent you choose
+It can save at most 1,000 data and output data by USB port
+Adapter: 0.5ml qPCR tube adapter                                                                                                                                                        0.2ml qPCR tube adapter                                                                                                                            
Two detection models of AE-Fluo-100

Code

Light

Excitation Filters

Emission Filters

AE-Fluo-100A

UV

365±20nm

420-480nm (60nm)

Blue

460±20nm

525-570nm (45nm)

AE-Fluo-100B

Blue

460±20nm

525-570nm (45nm)

Red

625±20nm

670-725nm (55nm)

AE-Fluo-100C

Blue

460±20nm

525-570nm (45nm)

Green

525±20nm

575-640nm (65nm)

 
 

Specification

Light source

LED

Dynamic range

Five orders of magnitude

Linear Dynamic Range

R2>0.995

Detector

Photodiode

Repeatability

<1.5%

Stability

<1.5%

Sensitivity

dsDNA: 0.5ng/ml

Measurement speed

3s (Once)

Dimension (W×D×H)mm

194 × 155 × 72.5 mm

Weight (kg)

0.4Kg

 

AE-Fluo-100 Quantitation kit

High sensitivity of dsDNA Quantitation Kit: 0.2--100ng High sensitivity of ssDNA Quantitation Kit: 1--200ng Broad range of dsDNA Quantitation Kit: 100--1600ng Protein Quantitation Kit is researching.

AE-Fluo-100 Quantitation kit operation

Channel

Excitation Filters

Normal kits

Applications

UV channel

365±20nm

Hoechst 33258, 4-MU, EnZCheK Caspase

Nucleic acid quantification, Plant GUS reporter gene detection, Poptosis detection

Blue channel

460±20nm

PicoGreen®, oligreen, RiboGreen®, GFP, Protein, Fluorescein, Quant-iT™

dsDNA, ssDNA, RNA quantification, GFP gene detection, Fluorescein detection, Protein detection

Green channel

525±20nm

Rhodamine, Cy3,

Rhodamine detection, Cy-3 fluorescence labeling detection, RFP gene detection, Cell activity position detection

RFP Vybrant Cytotoxicity

Red channel

625±20nm

Cy5, Quant-iT RNA

Cy-5 fluorescence labeling detection, RNA quantification

 
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